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insulin like growth factor ii igf2  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology insulin like growth factor ii igf2
    Characterization of pluripotent stem cell markers in iPSCs. (A) Immunofluorescence staining showing qualitative expression of pluripotency markers SOX2 and TRA-1-60 in iPSC cell lines N7, N9, N11, and N12. Scale bar=200 μm. (B) qRT-PCR analysis of gene expression levels of pluripotency markers NANOG and OCT4 in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). (C) Immunofluorescence staining showing qualitative expression of the hematopoietic commitment marker <t>IGF2</t> in the iPSC cell lines. Scale bar=200 μm. (D) qRT-PCR analysis of gene expression levels of the hematopoietic commitment marker IGF2 and its receptor IGF1R in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). iPSCs: induced pluripotent stem cells, IGF2: insulin-like growth factor II.
    Insulin Like Growth Factor Ii Igf2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/insulin+like+growth+factor+ii+igf2/IGF-II+Antibody/pmc12954770-35-20-25
    Average 93 stars, based on 47 article reviews
    insulin like growth factor ii igf2 - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator"

    Article Title: Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator

    Journal: International Journal of Stem Cells

    doi: 10.15283/ijsc25040

    Characterization of pluripotent stem cell markers in iPSCs. (A) Immunofluorescence staining showing qualitative expression of pluripotency markers SOX2 and TRA-1-60 in iPSC cell lines N7, N9, N11, and N12. Scale bar=200 μm. (B) qRT-PCR analysis of gene expression levels of pluripotency markers NANOG and OCT4 in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). (C) Immunofluorescence staining showing qualitative expression of the hematopoietic commitment marker IGF2 in the iPSC cell lines. Scale bar=200 μm. (D) qRT-PCR analysis of gene expression levels of the hematopoietic commitment marker IGF2 and its receptor IGF1R in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). iPSCs: induced pluripotent stem cells, IGF2: insulin-like growth factor II.
    Figure Legend Snippet: Characterization of pluripotent stem cell markers in iPSCs. (A) Immunofluorescence staining showing qualitative expression of pluripotency markers SOX2 and TRA-1-60 in iPSC cell lines N7, N9, N11, and N12. Scale bar=200 μm. (B) qRT-PCR analysis of gene expression levels of pluripotency markers NANOG and OCT4 in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). (C) Immunofluorescence staining showing qualitative expression of the hematopoietic commitment marker IGF2 in the iPSC cell lines. Scale bar=200 μm. (D) qRT-PCR analysis of gene expression levels of the hematopoietic commitment marker IGF2 and its receptor IGF1R in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). iPSCs: induced pluripotent stem cells, IGF2: insulin-like growth factor II.

    Techniques Used: Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Gene Expression, Marker

    Related Articles

    Immunofluorescence:

    Article Title: Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen-1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Article Title: Induced Pluripotent Stem Cells derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator.
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Staining:

    Article Title: Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen-1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Article Title: Induced Pluripotent Stem Cells derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator.
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Expressing:

    Article Title: Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen-1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Article Title: Induced Pluripotent Stem Cells derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator.
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Quantitative RT-PCR:

    Article Title: Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen-1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Article Title: Induced Pluripotent Stem Cells derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator.
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Gene Expression:

    Article Title: Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen-1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Article Title: Induced Pluripotent Stem Cells derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator.
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Marker:

    Article Title: Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen-1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.

    Article Title: Induced Pluripotent Stem Cells derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator.
    Article Snippet: Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.Following this, the cells were incubated with primary antibodies, followed by secondary antibodies.. The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).. Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.Nuclei were stained with 4’, 6-diamidino-2-phenylindole (DAPI) (VectorLabs, H-1200), and the cells were visualized using a fluorescent microscope (IX73 fluorescent microscope (Olympus) or LSM780 confocal microscope (Carl Zeiss) with LSM780 Image software.



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    Placentas were collected from gestation days (GD) 15 and 18 Ascl1 fl/fl and hep-Ascl1 -/- mice. (A) Frozen placental sections underwent periodic acid-Schiff (PAS) staining. Glycogen is stained red to purple. (B) Frozen placental sections were subjected to PL-I and PL-II in situ hybridization staining using RNAscope 2.5 HD Assay-BROWN kit. The PL-I and PL-II mRNAs are stained dark brown. (C) Quantification of relative intensity of Western blotting signals from . Data are presented as the mean fold changes relative to GD15 Ascl1 fl/fl mice (± SD; n = 3 for each group). *, P < 0.05, between Ascl1 fl/fl and hep-Ascl1 -/- mice. AKT, total protein kinase B; Ascl1 , achaete-scute homolog 1; ERK1/2, extracellular signal-regulated kinase 1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>IGF2,</t> insulin-like growth factor; PL-II, placental lactogen II.
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    Placentas were collected from gestation days (GD) 15 and 18 Ascl1 fl/fl and hep-Ascl1 -/- mice. (A) Frozen placental sections underwent periodic acid-Schiff (PAS) staining. Glycogen is stained red to purple. (B) Frozen placental sections were subjected to PL-I and PL-II in situ hybridization staining using RNAscope 2.5 HD Assay-BROWN kit. The PL-I and PL-II mRNAs are stained dark brown. (C) Quantification of relative intensity of Western blotting signals from . Data are presented as the mean fold changes relative to GD15 Ascl1 fl/fl mice (± SD; n = 3 for each group). *, P < 0.05, between Ascl1 fl/fl and hep-Ascl1 -/- mice. AKT, total protein kinase B; Ascl1 , achaete-scute homolog 1; ERK1/2, extracellular signal-regulated kinase 1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>IGF2,</t> insulin-like growth factor; PL-II, placental lactogen II.
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    Placentas were collected from gestation days (GD) 15 and 18 Ascl1 fl/fl and hep-Ascl1 -/- mice. (A) Frozen placental sections underwent periodic acid-Schiff (PAS) staining. Glycogen is stained red to purple. (B) Frozen placental sections were subjected to PL-I and PL-II in situ hybridization staining using RNAscope 2.5 HD Assay-BROWN kit. The PL-I and PL-II mRNAs are stained dark brown. (C) Quantification of relative intensity of Western blotting signals from . Data are presented as the mean fold changes relative to GD15 Ascl1 fl/fl mice (± SD; n = 3 for each group). *, P < 0.05, between Ascl1 fl/fl and hep-Ascl1 -/- mice. AKT, total protein kinase B; Ascl1 , achaete-scute homolog 1; ERK1/2, extracellular signal-regulated kinase 1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; <t>IGF2,</t> insulin-like growth factor; PL-II, placental lactogen II.
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    Image Search Results


    Characterization of pluripotent stem cell markers in iPSCs. (A) Immunofluorescence staining showing qualitative expression of pluripotency markers SOX2 and TRA-1-60 in iPSC cell lines N7, N9, N11, and N12. Scale bar=200 μm. (B) qRT-PCR analysis of gene expression levels of pluripotency markers NANOG and OCT4 in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). (C) Immunofluorescence staining showing qualitative expression of the hematopoietic commitment marker IGF2 in the iPSC cell lines. Scale bar=200 μm. (D) qRT-PCR analysis of gene expression levels of the hematopoietic commitment marker IGF2 and its receptor IGF1R in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). iPSCs: induced pluripotent stem cells, IGF2: insulin-like growth factor II.

    Journal: International Journal of Stem Cells

    Article Title: Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator

    doi: 10.15283/ijsc25040

    Figure Lengend Snippet: Characterization of pluripotent stem cell markers in iPSCs. (A) Immunofluorescence staining showing qualitative expression of pluripotency markers SOX2 and TRA-1-60 in iPSC cell lines N7, N9, N11, and N12. Scale bar=200 μm. (B) qRT-PCR analysis of gene expression levels of pluripotency markers NANOG and OCT4 in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). (C) Immunofluorescence staining showing qualitative expression of the hematopoietic commitment marker IGF2 in the iPSC cell lines. Scale bar=200 μm. (D) qRT-PCR analysis of gene expression levels of the hematopoietic commitment marker IGF2 and its receptor IGF1R in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). iPSCs: induced pluripotent stem cells, IGF2: insulin-like growth factor II.

    Article Snippet: The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen-1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).

    Techniques: Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Gene Expression, Marker

    Expression of miR-125b-5p and insulin-like growth factor 2 in vitro and in vivo . A: Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-125b-5p in AR42J cell lines in activated and inactive state; B: RT-qPCR was used to detect the expression of insulin-like growth factor 2 (IGF2) in AR42J cell lines under activated and inactive state; C: Western blot was used to detect the expression level of IGF2, and β-actin was used as the internal reference; D: RT-qPCR was used to detect the expression of miR-125b-5p in normal pancreatic tissues and pancreatitis tissues; E: RT-qPCR was used to detect the expression of IGF2 in normal pancreatic tissues and pancreatitis tissues; F: The mRNA and protein expression of miR-125b-5p and IGF2 in 4 cases of pancreatitis tissues were detected by RT-qPCR and Western blot. Among them, sample 2 was used as the standard to calculate the fold change of miR-125b-5p expression in other samples by comparing the miR-125b-5p/U6 ratio in sample 2. Western blot was used to detect the expression level of IGF2, and β-actin was used as the internal reference. The experiment was repeated three times and is expressed as mean ± SD; G: miR-125b-5p was negatively correlated with IGF2 protein expression level, a P < 0.05, b P < 0.005. AP: Acute pancreatitis; IGF2: Insulin-like growth factor 2; N: Normal.

    Journal: World Journal of Gastrointestinal Surgery

    Article Title: Acinous cell AR42J-derived exosome miR125b-5p promotes acute pancreatitis exacerbation by inhibiting M2 macrophage polarization via PI3K/AKT signaling pathway

    doi: 10.4240/wjgs.v15.i4.600

    Figure Lengend Snippet: Expression of miR-125b-5p and insulin-like growth factor 2 in vitro and in vivo . A: Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-125b-5p in AR42J cell lines in activated and inactive state; B: RT-qPCR was used to detect the expression of insulin-like growth factor 2 (IGF2) in AR42J cell lines under activated and inactive state; C: Western blot was used to detect the expression level of IGF2, and β-actin was used as the internal reference; D: RT-qPCR was used to detect the expression of miR-125b-5p in normal pancreatic tissues and pancreatitis tissues; E: RT-qPCR was used to detect the expression of IGF2 in normal pancreatic tissues and pancreatitis tissues; F: The mRNA and protein expression of miR-125b-5p and IGF2 in 4 cases of pancreatitis tissues were detected by RT-qPCR and Western blot. Among them, sample 2 was used as the standard to calculate the fold change of miR-125b-5p expression in other samples by comparing the miR-125b-5p/U6 ratio in sample 2. Western blot was used to detect the expression level of IGF2, and β-actin was used as the internal reference. The experiment was repeated three times and is expressed as mean ± SD; G: miR-125b-5p was negatively correlated with IGF2 protein expression level, a P < 0.05, b P < 0.005. AP: Acute pancreatitis; IGF2: Insulin-like growth factor 2; N: Normal.

    Article Snippet: After adding primary antibodies against CD9 (Abcam, Cambridge, United Kingdom), CD81 (Abcam, Cambridge, United Kingdom), CD63 (Abcam, Cambridge, United Kingdom), TSG101 (Protein Tech Group, IL, United States), CD206 (Protein Tech Group, IL, United States), inducible nitric oxide synthase (iNOS) (Abcam, Cambridge, United Kingdom), insulin-like growth factor 2 (IGF2) (Protein Tech Group, IL, United States), Bax (Protein Tech Group, IL, United States), Bcl-2 (Protein Tech Group, IL, United States), HMGB1 (Protein Tech Group, IL, United States), PI3K (Protein Tech Group, IL, United States), p-PI3K (Protein Tech Group, IL, United States), AKT (Protein Tech Group, IL, United States), p-AKT (Protein Tech Group, IL, United States), β-tubulin (Protein Tech Group, IL, United States), β-actin (Protein Tech Group, IL, United States), and GAPDH (Abcam, ab8245, 1:1000), the membrane was incubated at 4 °C overnight.

    Techniques: Expressing, In Vitro, In Vivo, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

    miR-125b-5p overexpression could promote necrosis and apoptosis of AR42J cells in the activated state. A: miR-125b-5p overexpression promoted the necrosis of AR42J cells in the activated state; B: Flow cytometry analysis confirmed that the percentage of cells in G0/G1 phase increased in AR42J cells in the activated state treated with the miR-125b-5p overexpression group; C: The percentage of apoptotic cells increased as confirmed by flow cytometry; D and E: The expression of insulin-like growth factor 2, BAX, Bcl-2 and HMGB-1 was confirmed by Western blot, a P < 0.05, c P < 0.0005. AP: Acute pancreatitis; IGF2: Insulin-like growth factor 2; N: Normal; NC: Negative control.

    Journal: World Journal of Gastrointestinal Surgery

    Article Title: Acinous cell AR42J-derived exosome miR125b-5p promotes acute pancreatitis exacerbation by inhibiting M2 macrophage polarization via PI3K/AKT signaling pathway

    doi: 10.4240/wjgs.v15.i4.600

    Figure Lengend Snippet: miR-125b-5p overexpression could promote necrosis and apoptosis of AR42J cells in the activated state. A: miR-125b-5p overexpression promoted the necrosis of AR42J cells in the activated state; B: Flow cytometry analysis confirmed that the percentage of cells in G0/G1 phase increased in AR42J cells in the activated state treated with the miR-125b-5p overexpression group; C: The percentage of apoptotic cells increased as confirmed by flow cytometry; D and E: The expression of insulin-like growth factor 2, BAX, Bcl-2 and HMGB-1 was confirmed by Western blot, a P < 0.05, c P < 0.0005. AP: Acute pancreatitis; IGF2: Insulin-like growth factor 2; N: Normal; NC: Negative control.

    Article Snippet: After adding primary antibodies against CD9 (Abcam, Cambridge, United Kingdom), CD81 (Abcam, Cambridge, United Kingdom), CD63 (Abcam, Cambridge, United Kingdom), TSG101 (Protein Tech Group, IL, United States), CD206 (Protein Tech Group, IL, United States), inducible nitric oxide synthase (iNOS) (Abcam, Cambridge, United Kingdom), insulin-like growth factor 2 (IGF2) (Protein Tech Group, IL, United States), Bax (Protein Tech Group, IL, United States), Bcl-2 (Protein Tech Group, IL, United States), HMGB1 (Protein Tech Group, IL, United States), PI3K (Protein Tech Group, IL, United States), p-PI3K (Protein Tech Group, IL, United States), AKT (Protein Tech Group, IL, United States), p-AKT (Protein Tech Group, IL, United States), β-tubulin (Protein Tech Group, IL, United States), β-actin (Protein Tech Group, IL, United States), and GAPDH (Abcam, ab8245, 1:1000), the membrane was incubated at 4 °C overnight.

    Techniques: Over Expression, Flow Cytometry, Expressing, Western Blot, Negative Control

    miR-125b-5p promotes acute pancreatitis exacerbation by inhibiting insulin-like growth factor 2 protein expression in PI3K/AKT signaling pathway. A: The potential binding sites of miR-125b-5p in the 3¢-untranslated regions (UTR) of wild-type insulin-like growth factor 2 (IGF2) (red part). The blue section is the mutation site in mutant IGF2 3¢-UTR sequence; B: Luciferase activity in IGF2 3¢-UTR of wild-type and mutant was detected after transfection of miR-125b-5p mimics and negative control miRNAs. The normalized luciferase activity of the transfected control miRNA was set to a relative luciferase activity of 1; C: The protein expression of PI3K/AKT signaling pathway was confirmed by western blot. a P < 0.05, b P < 0.005. AP: Acute pancreatitis; IGF2: Insulin-like growth factor 2; N: Normal; NC: Negative control; WT: Wild-type; MUT: Mutant; UTR: Untranslated regions; CDS: Coding sequence.

    Journal: World Journal of Gastrointestinal Surgery

    Article Title: Acinous cell AR42J-derived exosome miR125b-5p promotes acute pancreatitis exacerbation by inhibiting M2 macrophage polarization via PI3K/AKT signaling pathway

    doi: 10.4240/wjgs.v15.i4.600

    Figure Lengend Snippet: miR-125b-5p promotes acute pancreatitis exacerbation by inhibiting insulin-like growth factor 2 protein expression in PI3K/AKT signaling pathway. A: The potential binding sites of miR-125b-5p in the 3¢-untranslated regions (UTR) of wild-type insulin-like growth factor 2 (IGF2) (red part). The blue section is the mutation site in mutant IGF2 3¢-UTR sequence; B: Luciferase activity in IGF2 3¢-UTR of wild-type and mutant was detected after transfection of miR-125b-5p mimics and negative control miRNAs. The normalized luciferase activity of the transfected control miRNA was set to a relative luciferase activity of 1; C: The protein expression of PI3K/AKT signaling pathway was confirmed by western blot. a P < 0.05, b P < 0.005. AP: Acute pancreatitis; IGF2: Insulin-like growth factor 2; N: Normal; NC: Negative control; WT: Wild-type; MUT: Mutant; UTR: Untranslated regions; CDS: Coding sequence.

    Article Snippet: After adding primary antibodies against CD9 (Abcam, Cambridge, United Kingdom), CD81 (Abcam, Cambridge, United Kingdom), CD63 (Abcam, Cambridge, United Kingdom), TSG101 (Protein Tech Group, IL, United States), CD206 (Protein Tech Group, IL, United States), inducible nitric oxide synthase (iNOS) (Abcam, Cambridge, United Kingdom), insulin-like growth factor 2 (IGF2) (Protein Tech Group, IL, United States), Bax (Protein Tech Group, IL, United States), Bcl-2 (Protein Tech Group, IL, United States), HMGB1 (Protein Tech Group, IL, United States), PI3K (Protein Tech Group, IL, United States), p-PI3K (Protein Tech Group, IL, United States), AKT (Protein Tech Group, IL, United States), p-AKT (Protein Tech Group, IL, United States), β-tubulin (Protein Tech Group, IL, United States), β-actin (Protein Tech Group, IL, United States), and GAPDH (Abcam, ab8245, 1:1000), the membrane was incubated at 4 °C overnight.

    Techniques: Expressing, Binding Assay, Mutagenesis, Sequencing, Luciferase, Activity Assay, Transfection, Negative Control, Control, Western Blot

    miR125b-5p promotes the aggravation of inflammation in vivo . A: Intraperitoneal condition of rats in acute pancreatitis (AP) group and overexpression group; B: Ascites volume of rats in the three groups. The expression of miR-125b-5p in pancreatic tissues of sham group, AP group and overexpression group was detected by quantitative real-time polymerase chain reaction; C: The level of interleukin-6, tumor necrosis factor-alpha, C-reactive protein and reactive oxygen species was determined by enzyme linked immunosorbent assay; D: Histopathological image of rat pancreas in three groups; E: Pancreatic histopathology score and dry/wet ratio; F: Western blot showing the expression of BAX, Bcl-2, HMGB-1 and insulin-like growth factor 2 in the pancreatic tissues; G: Western blot showing the expression of p-PI3K, PI3K, p-AKT and AKT in the pancreatic tissues, a P < 0.05, b P < 0.005, c P < 0.0005. IGF2: Insulin-like growth factor 2; TNF-α: Tumor necrosis factor-alpha; AP: Acute pancreatitis; IL: Interleukin; TNF-α: Tumor necrosis factor-alpha; CRP: C-reactive protein; ROS: Reactive oxygen species; NC: Negative control.

    Journal: World Journal of Gastrointestinal Surgery

    Article Title: Acinous cell AR42J-derived exosome miR125b-5p promotes acute pancreatitis exacerbation by inhibiting M2 macrophage polarization via PI3K/AKT signaling pathway

    doi: 10.4240/wjgs.v15.i4.600

    Figure Lengend Snippet: miR125b-5p promotes the aggravation of inflammation in vivo . A: Intraperitoneal condition of rats in acute pancreatitis (AP) group and overexpression group; B: Ascites volume of rats in the three groups. The expression of miR-125b-5p in pancreatic tissues of sham group, AP group and overexpression group was detected by quantitative real-time polymerase chain reaction; C: The level of interleukin-6, tumor necrosis factor-alpha, C-reactive protein and reactive oxygen species was determined by enzyme linked immunosorbent assay; D: Histopathological image of rat pancreas in three groups; E: Pancreatic histopathology score and dry/wet ratio; F: Western blot showing the expression of BAX, Bcl-2, HMGB-1 and insulin-like growth factor 2 in the pancreatic tissues; G: Western blot showing the expression of p-PI3K, PI3K, p-AKT and AKT in the pancreatic tissues, a P < 0.05, b P < 0.005, c P < 0.0005. IGF2: Insulin-like growth factor 2; TNF-α: Tumor necrosis factor-alpha; AP: Acute pancreatitis; IL: Interleukin; TNF-α: Tumor necrosis factor-alpha; CRP: C-reactive protein; ROS: Reactive oxygen species; NC: Negative control.

    Article Snippet: After adding primary antibodies against CD9 (Abcam, Cambridge, United Kingdom), CD81 (Abcam, Cambridge, United Kingdom), CD63 (Abcam, Cambridge, United Kingdom), TSG101 (Protein Tech Group, IL, United States), CD206 (Protein Tech Group, IL, United States), inducible nitric oxide synthase (iNOS) (Abcam, Cambridge, United Kingdom), insulin-like growth factor 2 (IGF2) (Protein Tech Group, IL, United States), Bax (Protein Tech Group, IL, United States), Bcl-2 (Protein Tech Group, IL, United States), HMGB1 (Protein Tech Group, IL, United States), PI3K (Protein Tech Group, IL, United States), p-PI3K (Protein Tech Group, IL, United States), AKT (Protein Tech Group, IL, United States), p-AKT (Protein Tech Group, IL, United States), β-tubulin (Protein Tech Group, IL, United States), β-actin (Protein Tech Group, IL, United States), and GAPDH (Abcam, ab8245, 1:1000), the membrane was incubated at 4 °C overnight.

    Techniques: In Vivo, Over Expression, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Histopathology, Western Blot, Negative Control

    Placentas were collected from gestation days (GD) 15 and 18 Ascl1 fl/fl and hep-Ascl1 -/- mice. (A) Frozen placental sections underwent periodic acid-Schiff (PAS) staining. Glycogen is stained red to purple. (B) Frozen placental sections were subjected to PL-I and PL-II in situ hybridization staining using RNAscope 2.5 HD Assay-BROWN kit. The PL-I and PL-II mRNAs are stained dark brown. (C) Quantification of relative intensity of Western blotting signals from . Data are presented as the mean fold changes relative to GD15 Ascl1 fl/fl mice (± SD; n = 3 for each group). *, P < 0.05, between Ascl1 fl/fl and hep-Ascl1 -/- mice. AKT, total protein kinase B; Ascl1 , achaete-scute homolog 1; ERK1/2, extracellular signal-regulated kinase 1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IGF2, insulin-like growth factor; PL-II, placental lactogen II.

    Journal: bioRxiv

    Article Title: Activation of proneuronal transcription factor Ascl1 in maternal liver ensures a healthy pregnancy

    doi: 10.1101/2021.04.27.441617

    Figure Lengend Snippet: Placentas were collected from gestation days (GD) 15 and 18 Ascl1 fl/fl and hep-Ascl1 -/- mice. (A) Frozen placental sections underwent periodic acid-Schiff (PAS) staining. Glycogen is stained red to purple. (B) Frozen placental sections were subjected to PL-I and PL-II in situ hybridization staining using RNAscope 2.5 HD Assay-BROWN kit. The PL-I and PL-II mRNAs are stained dark brown. (C) Quantification of relative intensity of Western blotting signals from . Data are presented as the mean fold changes relative to GD15 Ascl1 fl/fl mice (± SD; n = 3 for each group). *, P < 0.05, between Ascl1 fl/fl and hep-Ascl1 -/- mice. AKT, total protein kinase B; Ascl1 , achaete-scute homolog 1; ERK1/2, extracellular signal-regulated kinase 1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IGF2, insulin-like growth factor; PL-II, placental lactogen II.

    Article Snippet: Serum IGF2 levels were quantified using a 1/6 dilution with the Mouse IGF-2 ELISA Kit (Boster Biological Technology, EK0381) as per directions by the manufacturer and read with the SpectraMax M2e spectrophotometer using the SoftMax Pro 6 program.

    Techniques: Staining, In Situ Hybridization, RNAscope, HD Assay, Western Blot

    Maternal livers were collected and weighed from nonpregnant (NP) and gestation days (GD) 15 and 18 Ascl1 fl/fl and hep-Ascl1 -/- mice. (A) Hepatic Igf2 mRNA levels were measured using qRT-PCR and presented as the mean fold changes relative to NP controls ± SD (n = 4-5). (B) Western blotting was performed using liver lysates with an antibody against IGF2. (C) Igf2 in situ hybridization on liver sections. (D) IGF2 immunostaining. (E) Levels of hepatic Igf2 promoter-specific transcript variants were measured using qRT-PCR and presented as the mean fold changes relative to NP controls ± SD (n = 4-5). (F) Levels of IGF2 protein in serum were measured using ELISA and presented as the mean fold changes ± SD (n = 3-5). *, P < 0.05; **, P < 0.01; ***, P < 0.001. P0, placental-specific Igf2 promoter; P1-3, placental- and fetal liver-specific Igf2 promoter.

    Journal: bioRxiv

    Article Title: Activation of proneuronal transcription factor Ascl1 in maternal liver ensures a healthy pregnancy

    doi: 10.1101/2021.04.27.441617

    Figure Lengend Snippet: Maternal livers were collected and weighed from nonpregnant (NP) and gestation days (GD) 15 and 18 Ascl1 fl/fl and hep-Ascl1 -/- mice. (A) Hepatic Igf2 mRNA levels were measured using qRT-PCR and presented as the mean fold changes relative to NP controls ± SD (n = 4-5). (B) Western blotting was performed using liver lysates with an antibody against IGF2. (C) Igf2 in situ hybridization on liver sections. (D) IGF2 immunostaining. (E) Levels of hepatic Igf2 promoter-specific transcript variants were measured using qRT-PCR and presented as the mean fold changes relative to NP controls ± SD (n = 4-5). (F) Levels of IGF2 protein in serum were measured using ELISA and presented as the mean fold changes ± SD (n = 3-5). *, P < 0.05; **, P < 0.01; ***, P < 0.001. P0, placental-specific Igf2 promoter; P1-3, placental- and fetal liver-specific Igf2 promoter.

    Article Snippet: Serum IGF2 levels were quantified using a 1/6 dilution with the Mouse IGF-2 ELISA Kit (Boster Biological Technology, EK0381) as per directions by the manufacturer and read with the SpectraMax M2e spectrophotometer using the SoftMax Pro 6 program.

    Techniques: Quantitative RT-PCR, Western Blot, In Situ Hybridization, Immunostaining, Enzyme-linked Immunosorbent Assay